It can be used to separate proteins under non-denaturing conditions depending on Hydrophobic interaction chromatography (HIC) is a widely used chromatographic technique that finds application at different stages of downstream processing. John R. McDonald, Michael P. Walsh, in Calcium-Binding Proteins in Health and Disease, 1987Publisher Summary This chapter elaborates the Ca 2+ dependent hydrophobic interaction chromatography on phenyl-Sepharose CL-4B that has been used in the identification and isolation of a number of novel, heat-stable and low-molecular weight … Naturally, the most suitable method is hydrophobic interaction chromatography (HIC) (Drake et al., 2014; Godwin et al., 2015; Kudirka et al., 2015). 20529, F-60205 Compie`gne Cedex, France 2Pure and Applied Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, P.O. Contents lists available at ScienceDirect Analytica Chimica Acta ... Stationary and mobile phases in hydrophilic interaction chromatography: a review Pavel Jandera ... Hydrophobic interactions. 2. The hydrophobic nature of mixed-mode resins expressed has been exploited for aggregate removal during post-Protein Molecular A polishing [2,3]. It was first described by Shepard and Tiselius in 1949 using the term “salting out chromatography.” in 1949 using the term “salting out chromatography.” Predicting the behaviour of proteins in hydrophobic interaction chromatography: 2. Hydrophobic peptide tags as tools in bioseparation Sara Fexby1 and Leif Bu¨low2 1Present address: Laboratoire de Technologie Enzymatique, UMR 6022 du CNRS, Universite´ de Technologie de Compie`gne, B.P. Interaction-based purification tools, such as hydrophobic interaction chromatography (HIC) and ion exchange chromatography (IEC), are more popular methodology … C.T. Box 124, SE-221 00 Lund,
The salt independent binding behavior of and Queiroz, in Liquid Chromatography: Fundamentals and Instrumentation, 20136.1 Introduction In recent years hydrophobic interaction chromatography (HIC) has emerged as a powerful technique for purification of biological compounds on a laboratory scale as well as at the industrial scale. In theory, hydrophobic interaction chromatography (HIC) and RPLC are closely related, as in both techniques separation is based on hydrophobic interactions between the surface of an analyte and the stationary phase. ScienceDirect - Journal of Chromatography A, Volume 1107, Issues 1-2, Pages 1-310 (24 February 2006) 12. Hydrophobic interaction chromatography (HIC) is a high-resolution technique widely used for analysis and purification of biomolecules based on differences in their hydrophobicity. 10.2.2.1 Hydrophobic Interaction Chromatography. Hydrophobic interaction chromatography, biphasic partitioning, adsorption of probes, contact angle measurements, and X-ray photon correlation spectroscopy can be utilized for the determination of surface hydrophobicity [9][10]. …
The search has been done using as keywords [(Hydrophilic Interaction Chromatography) or (HILIC)] and [(Food) or (Beverages) or (Drinks) or (Vegetables) or (Fruits)]. However, in application the techniques are very different. Hydrophobic interaction chromatography (HIC) is emerging as a useful technique for the separation of biological compounds. Using a statistical description of their surface amino acid distribution • ARTICLE applications for mixed-mode chromatography have emerged, particularly modes involving a combination of hydrophobic and electrostatic The interactions. The most commonly used mixed-mode chromatography in bioseparations, hydrophobic-ionexchange chromatography, had been reported almost as early as hydrophobic interaction chromatography emerged (Er-el et al., 1972; Yon, 1972; Hofstee, 1973a,b; Shaltiel and Er-El, 1973). Hydrophilic interaction chromatography (HILIC) is valuable alternative to reversed-phase liquid chromatography separations of polar, weakly acidic or basic samples. Online coupling of analytical hydrophobic interaction chromatography with native mass spectrometry for the characterization of monoclonal antibodies and related products Author links open overlay panel Yuetian Yan Tao Xing Shunhai Wang Thomas J.
Tomaz, J.A. We describe a novel polymer-based zwitterionic stationary phase for hydrophilic interaction chromatography (HILIC). Hydrophobic interaction chromatography General rate model Preferential interaction isotherm abstract A general rate model was employed in concert with a preferential interaction quadratic adsorption isotherm for the characterization of HIC resins and the prediction of solute behavior in these separa-tion systems.