Amazon.com: Anion exchange protein 3 (SLC4A3), Monkey, ELISA Kit: Industrial & Scientific. View protein in InterPro IPR001717 Anion_exchange IPR002979 Anion_exchange_3 IPR018241 Anion_exchange_CS IPR013769 Band3_cytoplasmic_dom IPR011531 HCO3_transpt_C IPR003020 HCO3_transpt_euk IPR016152 PTrfase/Anion_transptr: PANTHER i: PTHR11453 PTHR11453, 1 hit PTHR11453:SF15 PTHR11453:SF15, 1 hit Band 3 protein is a typical polytropic membrane protein and mediates the exchange of the cellular HCO3- with CI- in plasma, which has been known as the "Chloride Shift". Try Prime Industrial & Scientific Go Search EN Hello, Sign in … The transport rate was low, as expected from the model, which ERYTHROCYTE ANION EXCHANGE AND THE BAND 3 PROTEIN 269 suggests that the flow of C,CI, is less than 0.1% of the total monovalent anion exchange (Gunn, 1972). AE2 is functionally similar to the Band 3 Cl − /HCO3 − exchange protein. In order to elute an unknown protein via ion exchange, a gradient of different concentrations of salt with appropriate buffer is required to test at which salt concentration our desired protein is eluted.
Owing to the "Chloride Shift", red blood cells can discriminate the metabolically active cells from inactive cells and deliver oxygen particularly to metabolically active tissues that produce carbon dioxide. Anion exchange chromatography is a form of ion exchange chromatography (IEX), which is used to separate molecules based on their net surface charge. Plasma membrane anion exchange protein of wide distribution. QIAGEN-tips contain a unique, patented anion-exchange resin which eliminates the need for expensive equipment and reagents such as ultracentrifuges, HPLC/FPLC‚ or CsCl. Skip to main content.
3.41.3.4.4 High-pH anion exchange chromatography High-pH anion exchange chromatography (HPAEC) involves a high-pH separation of derivatized and underivatized oligosaccharides [51, 52] . Ion exchange protein purification is possible because most proteins bear nonzero net electrostatic charges at all pHs except at pH=pI (isoelectric point). Skip to main content. Anion Exchange through Band 3 Protein in Di erent Oxidative Experimental Conditions and the Role of Antioxidants Oxidant molecules, transferred by the blood stream, exert their action on the cell membrane with possible e ects on transport systems and, in … Amazon.com: Anion exchange protein 3 (SLC4A3), Sheep, ELISA Kit: Industrial & Scientific.
The Protein-Pak HR material is stable at pH values from 2 to 12 and withstands exposure to caustic solutions. Same detectors (miniDAWN TREOS, Optilab T-rEX and AKTA explorer system with a UV-900 detector) were used in-line with an anion exchange chromatography analytical … Anion exchange protein 2 (AE2) is a membrane transport protein that in humans is encoded by the SLC4A2 gene. Both BAE3 and CAE3 forms transport Cl(-). Try Prime EN Hello, Sign in Account & Lists Sign in Account & Lists Returns & Orders Try Prime Cart. Toxic and mutagenic substances such as phenol, chloroform, and ethidium bromide are also not required. In the case of anion exchange, DEAE is used to pack our column. Protein Purification by Ion-Exchange Chromatography. Protein-Pak HR Ion-Exchange Glass Columns The naturally hydrophilic polymer reduces non-specific adsorption, resulting in quantitative recovery of proteins while maintaining bioactivity.
Anion exchange chromatography, more specifically, uses a positively charged ion exchange resin with an affinity for molecules having net negative surface charges. Industrial & Scientific.