how to measure zone of inhibition

5.1 to 7.6 cm] above, a black, nonreflecting, flat surface, illuminatedby a reflectedlight source. The zone of inhibition can be used to measure the susceptibility of the bacteria to wards the antibiotic. Weconcluded that zone diameters should be measured from the back ofthe plate while it is resting on, or held 2 to 3 inches [ca. The process of measuring the diameter of this Zone of Inhibition can be automated using Image processing. Take your measurement in millimeters.

How to Find the Zone of Inhibition ● In this method, pure culture of the bacterium is swabbed on a plate of sterile Mueller-Hinton agar. Measure from the center of the disk to the edge of area with zero growth. In this work an algorithm is developed, using Computer Vision, which will detect the zones of inhibition of the bacteria. This is called a zone of inhibition. If antimicrobial agent leaches from the object into the agar and then exerts a growth-inhibiting effect, then a clear zone (the zone of inhibition) appears around the test product. The zone is measured by the diameter (across the zone) and you do not consider the disc at all.

The size of the zone of inhibition is usually related to the level of antimicrobial activity present in the sample or product - a larger zone of inhibition usually means that the antimicrobial is more potent. Take a ruler or caliper that measures in millimeters and place the "0" in the center of the antibiotic disk. Another factor that will influence the size of a zone is the diffusion of the antibiotic within the agar medium and varies based on the molecular configuration of the antibiotic. The size of this zone depends on many factors, one being how effective the antibiotic is at stopping the growth of the bacterium.

● Then a disk impregnated with the antibiotic to be tested is placed on it. To measure the zone of inhibition, first place the plate on a non-reflective surface.